Introduction of the NWSL to Louisville, which has experienced immense success on the pitch already with two-time USL Championship winner Louisville City FC, will bring to Lynn Family Stadium household names from across the globe.

OL Reign is an American professional women's soccer team based in Seattle, Washington. In 2020, OL Groupe, the parent company of French clubs Olympique Lyonnais and Olympique Lyonnais Féminin, became the team's majority owner and rebranded to its current name, badge, and colors. OL Reign announced that Lumen Field will become its home stadium beginning in 2022. Lumen Field has a capacity of 68,740.

Club América Femenil is a Mexican women's football club based in Mexico City. The club has been the female section of Club América since 2017. The team plays in the Liga MX Femenil, which began in 2017. Club America’s only title came during the Apertura 2018 Tournament.

Tokyo Verdy Beleza was founded in 1981. This powerhouse is the only Japanese women's club to have never been relegated. NTV Beleza has attained the domestic treble (winning the Nadeshiko League, League Cup, and Empress's Cup) three times in a row! 2007, 2018 and 2019.

Associazione Calcio Milan, also known as Milan Women , is an Italian women's association football club affiliated with A.C. Milan. The team started playing in Seria A in the 2018–19 season. Milan's first season saw the team finish in 3rd place. This world-renowned team is taking over the Seria A, and now is excited to showcase their skills at the 2022 edition of The Women’s Cup.

Tottenham Hotspur F.C. Women is an English women's football club affiliated with Tottenham Hotspur, that currently plays in the FA WSL. Tottenham Hotspur currently plays at The Hive Stadium located in Canons Park.